Coding
Part:BBa_K563012
Designed by: Boxuan Zeng Group: iGEM11_Tianjin (2011-10-05)
Tor2 with I1957T mutation
The Tor2 with I1957T mutation will resist to rapamycin. Its reaction to the KOG1 is increased. It has a higher kinase activity than tor2. In our project the
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 4657
Illegal EcoRI site found at 5077
Illegal EcoRI site found at 5360
Illegal EcoRI site found at 6236
Illegal XbaI site found at 2865
Illegal XbaI site found at 3810
Illegal XbaI site found at 5617
Illegal XbaI site found at 6232
Illegal XbaI site found at 7062
Illegal SpeI site found at 7014 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4657
Illegal EcoRI site found at 5077
Illegal EcoRI site found at 5360
Illegal EcoRI site found at 6236
Illegal NheI site found at 5457
Illegal NheI site found at 7374
Illegal SpeI site found at 7014 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4657
Illegal EcoRI site found at 5077
Illegal EcoRI site found at 5360
Illegal EcoRI site found at 6236
Illegal BglII site found at 2028
Illegal BglII site found at 7296
Illegal BamHI site found at 5643 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 4657
Illegal EcoRI site found at 5077
Illegal EcoRI site found at 5360
Illegal EcoRI site found at 6236
Illegal XbaI site found at 2865
Illegal XbaI site found at 3810
Illegal XbaI site found at 5617
Illegal XbaI site found at 6232
Illegal XbaI site found at 7062
Illegal SpeI site found at 7014 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 4657
Illegal EcoRI site found at 5077
Illegal EcoRI site found at 5360
Illegal EcoRI site found at 6236
Illegal XbaI site found at 2865
Illegal XbaI site found at 3810
Illegal XbaI site found at 5617
Illegal XbaI site found at 6232
Illegal XbaI site found at 7062
Illegal SpeI site found at 7014
Illegal NgoMIV site found at 4996
Illegal AgeI site found at 4236
Illegal AgeI site found at 7393 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1475
Illegal SapI.rc site found at 4666
Illegal SapI.rc site found at 4918
[edit]
Categories
Parameters
n/a | Tor2 with I1957T mutation |